BAR-ILAN INSTITUTE OF NANOTECHNOLOGY & ADVANCED MATERIALS | 2019 ANNUAL REPORT

57 detection of troponin levels early after the onset of symptoms significantly reduces the time required to diagnose or rule out heart attack. Today, most high sensitivity laboratory devices cannot provide results in a clinically relevant time frame, and, unfortunately, present point-of-care (POC) devices are substantially less sensitive than laboratory assays. To reap the benefits of improved patient care and reduced costs through prompt diagnostics, new platforms must be developed. We have developed a novel magnetic modulation biosensing (MMB) technology that can rapidly detect very low concentrations of biomarkers, such as proteins, antibodies, specific DNA sequences, and others. By combining magnetic and fluorescent labeling of target analytes and then applying an alternating external magnetic field gradient, we can significantly increase the sensitivity of fluorescence detection. The external alternating magnetic field condenses the targets to a small volume and imposes on them a periodic motion, which enables separation of the background signal from the oscillating target signal without complex sample preparation. The MMB system can detect fM concentrations of specific DNA sequences, as little as 0.08 ng/L of the human protein Interluekin-8 (IL- 8) in plasma, or 100 ng/L of Zika virus IgM and IgG antibodies in serum. Improving the Sensitivity of Fluorescence- based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads Publications 2018 and 2019 • Shira Roth, Orr Hadass, Meir Cohen, Jasenka Verbarg, Jennifer Wilsey, and Amos Danielli, “Improving the Sensitivity of Fluorescence-Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads”, Small, 15, 1803751, 2019 . • Shmuel Burg, Meir Cohen, Michael Margulis, Shira Roth, and Amos Danielli, “Magnetically aggregated biosensors for sensitive detection of biomarkers at low concentrations”, Appl. Phys. Lett. 115, 103702, 2019 . • Moshe Stern, Meir Cohen and Amos Danielli, “Configuration and Design of Electromagnets for Rapid and Precise Manipulation of Magnetic Beads in Biosensing Applications”, Micromachines, 10(11), 784, 2019 . • Michael Margulis, Saar Ashri, Meir Cohen, Amos Danielli, “Detecting nucleic acid fragments in serum using a magneticallymodulated sandwich assay”, J. Biophotonics, 12:c201900104, 2019 . • S Roth, O Hadass, M Cohen, J Verbarg, J Wilsey, A Danielli, “Improving the Sensitivity of Fluorescence-Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads”, Small, 1803751, 2018 . • Y Michelson, Y Lustig, S Avivi, E Schwartz, A Danielli, “Highly sensitive and specific Zika virus serological assays using a magnetic modulation biosensing system”, The Journal of infectious diseases, 2018 . Prof. Moshe Deutsch Department of Physics Member of BINA, Nano-Materials & Nano-Energy Center Research Areas • Experimental x-ray studies of surface properties of liquid metals • Simple liquids and liquid crystals • Structure of mono-molecular Langmuir films • Dynamical x-ray diffraction phenomena in perfect crystals • High precision crystal binding studies • X-ray emission and absorption spectroscopy • Synchrotron radiation and its applications Publications 2018 and 2019 • Diego Pontoni, Julia Haddad, Bridget M Murphy, Sven Festersen, Oleg Konovalov, Benjamin M Ocko, Moshe Deutsch, “Surface Phases and Surface Freezing in an Ionic Liquid “, The Journal of Physical Chemistry C, 123 (5), 3058-3066, 2019 . • Orlando Marin, Maria Alesker, Shani Guttman, Gregory Gershinsky, Eitan Edri, HagayS hpaisman, Rodrigo E. Guerra, David Zitoun, Moshe Deutsch, Eli Sloutskin, “Self-faceting of emulsion droplets as a route to solid icosahedra and other polyhedral”, Journal of Colloid and Interface Science Volume 538, Pages 541-545, 2019 . • Shani Guttman, Ellina Kesselman, Avi Jacob, Orlando Marin, Dganit Danino, Moshe Deutsch, and Eli Sloutskin, “Nanostructures, Faceting, and Splitting