BAR-ILAN INSTITUTE OF NANOTECHNOLOGY & ADVANCED MATERIALS | 2019 ANNUAL REPORT

Prof. Patrick Sebbah Department of Physics Member of BINA, Nano-Photonics Center Research Areas • Experimental physics in Wave Propagation in Complex Media, • Light-Matter Interaction • Elastic waves in structures plates • Multiple Scattering, Anderson Localization • Nonlinear and Active Random Media, Random Lasers • Nonlinear Scattering, Instabilities • Speckle Statistics, Optical Singularities • Metamaterials • Microwave scattering and localization in disordered system. 84 • M Galanty, O Shavit, A Weissman, H Aharon, D Gachet, E Segal, A Salomon, “Second harmonic generation hotspot on a centrosymmetric smooth silver surface”, Light: Science & Applications 7 (1), 49, 2018 . Prof. Ronit Sarid The Mina & Everard Goodman Faculty of Life Sciences Member of BINA , Nano-Medicine Center Research Areas • The study of the etiology of cancer is critical to understanding how to prevent this disease and to improve treatment. • Exploring the Cellular and Viral Pathways of KSHV Infection Cycle and Pathogenesis • KSHV: Clinical and Epidemiological Studies • Drug Discovery, Design and Delivery Research for Infectious Disease Abstract Human Herpesviruses KSHV, also referred to as human herpesvirus-8 (HHV-8), is one the few viruses proven to be associated with tumorigenesis in humans. Its causal association with all clinical and epidemiological variants of Kaposi’s sarcoma (KS) is well established. KS is found frequently in AIDS patients, but also has other variants including African, iatrogenic and classic KS. Classic KS affects apparently immunocompetent individuals, mostly men of Mediterranean or Eastern European origin. Of note, in Israel the incidence rate of the classic KS variant is one of the highest in the developed world. KSHV is also involved in the pathogenesis of two lymphoproliferative disorders: primary effusion lymphoma (PEL) and a subset of multicentric Castleman’s disease (MCD). While KSHV is important from clinical and public health standpoints, it also provides a source of basic information on how viruses might induce cellular proliferation and transformation and also offers clues for the understanding of normal and tumorigenic signal transduction pathways. HSV-1, another human herpesvirus, is a common infectious agent that occurs worldwide and infects humans of all ages. The outcome of HSV-1 infection includes a wide variety of clinical manifestations, ranging from asymptomatic infection to oral cold sores and severe encephalitis. Our studies involve five major areas: 1. Viral gene culprits: Characterization of viral genes and their protein products involved in KSHV pathogenesis. This includes the construction of recombinant viruses encoding specific mutations or tagged viral genes. 2. Tracking virus entry, uncoating, assembly and egress by using recombinant viruses that express selected virion-associated proteins fused to fluorescent tags. 3. Tracking the nucleolar compartment and the involvement and modifications of nucleolar components during the infectious cycle. This includes examination of the effects of nucleolar stress on the infectious cycle. 4. Viral structure: Define how multiprotein virion assemblies associate and interact by analyzing isolated complexes and in the context of the virion shell. This could potentially lead to the discovery of novel approaches to inhibit virion disassembly and assembly for antiviral interventions. 5. Inhibition of virus infection by using composite nanoparticles or microspheres that block the attachment of the virions to host cells. Publications 2018 and 2019 • Dünn-Kittenplon, D.D., Kalt, I., Lellouche, J.M. and Sarid, R. “The KSHV portal protein ORF43 is essential for the production of infectious viral particles”. Virology 529:205-215, 2019 . • Rinne, S.J., Sipilä, L.J., Sulo, P., Jouanguy, E., Béziat, V., Abel, L., Casanova, J.L., Parvaneh, N., Balighi, K., Guttman- Yassky, E., Sarid, R., Aaltonen, L.A. and Aavikko M . “Candidate predisposition variants in Kaposi sarcoma as detected by whole-genome sequencing”. Open Forum Infectious Disease 17:ofz337, 2019 . (Selected as “Editor Choice” to be highlighted). • Gelgor, A., Gam ze Letova, C., Yegorov, Y., Kalt, I. and Sarid, R., “Nucleolar stress enhances lytic reactivation of the Kaposi’s sarcoma-associated herpesvirus”. Oncotarget 9:13822-13833, 2018 . • Calabrò., M. and Sarid, R., “HHV-8 and lymphoproliferative disorders” (Review). Mediterranean Journal of Hematology and Infectious Diseases 10:1e2018061, 2018 .