BAR-ILAN INSTITUTE OF NANOTECHNOLOGY & ADVANCED MATERIALS | 2019 ANNUAL REPORT

85 Prof. Amos Sharoni Department of Physics Member of BINA, Nano-Magnetism Center Research Areas • Phase transitions on the nano-scale • Spintronics • New Temperature Coefficient of Resistance (TCR) materials • Organic/SC hybrid Publications 2018 and 2019 • E Zion, N Haham, L Klein, A Sharoni, “Low temperature divergence in the AHE and AMR of ultra-thin Pt/Co/Pt trilayers”, Journal of Magnetism and Magnetic Materials 485, 314-319, 2019 . • S Harazi, O Kapon, A Sharoni, YR Tischler, “Direct Formation of Carbocyanine J-Aggregates in Organic Solvent”, The Journal of Physical Chemistry C 123 (31), 19087-19093, 2019 . • I Shlimak, E Zion, A Butenko, Y Kaganovskii, V Richter, A Sharoni, E Kogan, M Kaveh, “Irradiation-induced metal- insulator transition in monolayer graphene”, FlatChem Volume 14, 100084, 2019 . • K Baranes, D Hibsh, S Cohen, T Yamin, S Efroni, A Sharoni, O Shefi, “Comparing Transcriptome Profiles of Neurons Interfacing Adjacent Cells and Nanopatterned Substrates Reveals Fundamental Neuronal Interactions”, Nano letters 19 (3), 1451-1459, 2019 . • O. J. Sharon, A. Sharoni, J. Berger, A. Shaulov, and Y. Yeshurun, “Little-Parks oscillations in superconducting ring with Josephson junctions,” J. Phys. Conf. Ser., vol. 969, p. 12047, Mar. 2018 . • O Sharon, AA Shaulov, J Berger, A Sharoni, R Berkovits, Y Yeshurun, “Current-Induced Crossover of Flux Periodicity from h/2e to h/e in Superconducting Nb Nano-Ring”, Nano letters, 2018 . • M Marcus, A Smith, A Maswadeh, Z Shemesh, I Zak, M Motiei, H Schori, S Margel, A Sharoni, O Shefi, “Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles”, Nanomaterials 8 (9), 707, 2018 . Prof. Yaron Shav-Tal The Mina & Everard Goodman Faculty of Life Sciences Member of BINA, Nano-Medicine Center Research Areas • Gene expression control in live cells • Live-cell imaging and RNA dynamics • Nuclear structure and function Abstract Our studies focus on the processes of mRNA transcription, trafficking, and export from the nucleus. We use cell systems in which major elements of the gene expression pathway are fluorescently tagged and visualized in real-time, in single living cells. We are looking for a motivated and experimentally qualified postdoc for a study focused on mRNA export in mammalian cells. Prior experience in imaging and image analysis is required. The distribution of splicing factors (green) a human nucleus shows their localization in nuclear speckles and at the site of an active gene (yellow). Publications 2018 and 2019 • Hochberg-Laufer H, Schwed-Gross A, Neugebauer KM, and Shav-Tal Y, “Uncoupling of nucleo-cytoplasmic RNA export and localization during stress”, Nucleic Acids Res. 47, 4778-4797. 2019 . • Ben-Yishay R, and Shav-Tal Y. “The dynamic lifecycle of mRNA in the nucleus”, Curr. Opin. Cell Biol. 58, 69-75. 2019 . • Hochberg-Laufer H, and Shav-Tal Y. “Active RNA polymerase II curbs chromatin movement”, J. Cell Biol. 218, 1427-1428. 2019 . • Saady A, Böttner V, Meng M, Varon E, Shav-Tal Y, Ducho C, and Fischer B., “An oligonucleotide probe incorporating the chromophore of Green Fluorescent Protein is useful for the detection of HER-2 mRNA breast cancer marker”, Euro. J. Med. Chem. 173, 99-106. 2019 . • Greenberg E, Hochberg-Laufer H, Blanga S, Kinor N, and Shav-Tal Y., “Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization” Nucleic Acids Res. 47, e109. 2019 . • Ben-Yishay R, Mor A, Shraga A, Ashkenazy- Titelman A, Kinor N, Schwed-Gross A, Jacob A, Kozer N, Kumar P, Garini Y, and Shav-Tal Y., “Imaging within single NPCs reveals NXF1’s role in mRNA export on the cytoplasmic side of the pore”, J. Cell Biol. 218, 2962-2981. 2019 . • Ben-Yishay R, and Shav-Tal Y., “Detection of mRNAs anchored to the nuclear envelope during export inhibition in living cells”. In: Methods in Molecular Biology. Ed: Shav-Tal Y. Humana Press Inc., Totowa, NJ. Vol.2038, pp 151-163. 2019 . • Hochberg-Laufer H, Neufeld N, Brody Y, Nadav S, Ben-Yishay R, and Shav-Tal Y., “Availability of splicing factors in the nucleoplasm can regulate the release of mRNA from the gene after transcription”. PLoS Genet. In press. 2019 . • T Tripathi, J Prakash, Y Shav-Tal, “Phospho- Tau Impairs Nuclear-Cytoplasmic Transport”, ACS chemical neuroscience, 10, 36-38, 2018 . • S Yunger, P Kafri, L Rosenfeld, E Greenberg, N Kinor, Y Garini, Y Shav-Tal, “S-phase transcriptional buffering quantified on two different promoters”, Life science alliance 1 (5), e201800086, 2018 . • Y Shav-Tal, T Tripathi, “Yeast and Human Nuclear Pore Complexes: Not So Similar After All”, Trends in Cell Biology, 28, 589- 591, 2018 . Books Invited book editor: Methods in Molecular Biology: Imaging Gene Expression. Ed: Shav- Tal Y. Humana Press Inc., Totowa, NJ. Vol 2038. Contains 17 chapters. In press. 2019 .