BAR-ILAN INSTITUTE OF NANOTECHNOLOGY & ADVANCED MATERIALS | 2019 ANNUAL REPORT

91 Abstract Action Spectroscopy We study biochromophores in the gas phase in order to understand the quantum molecular basis for phenomena such as vision and photosynthesis. For this purpose we employ and develop techniques which include ion traps and ion mobility spectroscopy. Publications 2018 and 2019 • A Ben Yaacov, L Chen, L Musbat, M Nihamkin, A Kaiser, Y Toker, “Clusters of betaine with positive and negative ions: Evidence for the betaine tetramer being magic”, The Journal of chemical physics 151 (18), 184303, 2019 . • M Nihamkin, A Kaiser, I Nemtsov, P Martini, P Scheier, Y Mastai, Y Toker, “Chiral recognition via abundances of mixed chiral clusters”, International Journal of Mass Spectrometry 446, 116215, 2019 . • H. R. and D. E. C. Tarick J. El-Baba, a Daniel R. Fuller, a Daniel W. Woodall, a Shannon A. Raab, a Christopher R. Conant, a Jonathan M. Dilger, b Yoni Toker, c Evan R. Williams, d David H. Russelle and David E. Clemmer Tarick J. El-Baba, a Daniel R. Fuller, a Daniel W. W, “Melting proteins confined in nanodroplets with 10.6 μm light provides clues about early steps of denaturation,” Chem. Commun., 2018 . • L Musbat, S Assis, JM Dilger, TJ El-Baba, DR Fuller, JL Knudsen, HV Kiefer, A Hirshfeld, N Friedman, LH Andersen, M Sheves, DE Clemmer, Y Toker, “Action and Ion Mobility Spectroscopy of a Shortened Retinal Derivative”, Journal of The American Society for Mass Spectrometry 29 (11), 2152-2159, 2018 . • Y Toker, J Langeland, E Gruber, C Kjær, SB Nielsen, LH Andersen, VA Borin, I Schapiro, “Counterion-controlled spectral tuning of the protonated Schiff-base retinal”, Physical Review A 98 (4), 043428, 2018 . Dr. Amit Tzur The Mina & Everard Goodman Faculty of Life Sciences Member of BINA, Nano-Medicine Center Research Areas • The mammalian cell cycle • Cell growth and size homeostasis of proliferating cells Publications 2018 and 2019 • H Nevenzal, M Noach-Hirsh, O Skornik- Bustan, L Brio, E Barbiro-Michaely, Y Glick, D Avrahami, R Lahmi, A Tzur, D Gerber, “A high-throughput integrated microfluidics method enables tyrosine autophosphorylation discovery”, Communications biology 2 (1), 42, 2019 . Prof. ShimonWeiss Department of Physics BIU Department of Chemistry and Biochemistry UCLA Member of BINA, Nano-Medicine & Nano-Photonic Center Research Areas • Single molecule detection and spectroscopy, • Dynamic structural/molecular biology, • Protein folding, protein-protein and protein-DNA interactions, • Novel Bio-Nano-Technology probes, semiconductor nanocrystals, quantum dots, semiconductor nanocrystals voltage sensors, • Fluorescence microscopy/spectroscopy, super resolution microscopy, • Mesoscopic systems Abstract Voltage Nanosensors for brain research We are developing targetable voltage sensing inorganic nanoparticles (vsNPs) that are designed to self-insert into the cell membrane and non-invasively optically record, via the quantum confined Stark effect, action potential on the single- particle level, at multi-sites and in a large field-of-view. We have synthesized a library of vsNPs with different compositions and developed a high-throughput screen for optimization of their performance. We are exploring several strategies for imparting these vsNPs with membrane-protein-like properties, including functionalization with libraries of peptides, lipids, and nanodiscs. We are developing screening assays for improving the efficiency of vsNPs’ membrane insertion and for their voltage sensitivity once embedded in the membrane. These novel voltage nansensors hold great promise for electrophysiological investigations of the nervous system. An illustration of a neuron labeled with single voltage sensing nanoparticles (green) for high resolution spatial and temporal membrane potential measurements. Publications 2018 and 2019 • A Grupi, I Ashur, N Degani ѧ Katzav, S Yudovich, Z Shapira, A Marzouq, L Morgenstein, Y Mandel, S Weiss, “Interfacing the Cell with “Biomimetic Membrane Proteins”, Small, 15, 1903006, 2019 . • S Yudovich, L Shani, A Grupi, O Bar-Elli, D Steinitz, D Oron, S Weiss, “Ratiometric widefield imaging with spectrally balanced detection”, Biomedical optics express 10 (10), 5385-5394, 2019 . • MK Paul, A Basu, B Bisht, P Pagano, Y Fontebasso, K Krysan, L Tran, M Alioscha- Perez, J Minna, D DiCarlo, H Sahli, S Weiss, SM Dubinett, “Regulation and role of actin dynamics in promoting premalignant cell migration”, Cancer Research 79 (13 Supplement), 1030-1030, 2019 .