2021 ANNUAL REPORT | Bar-Ilan Institute of Nanotechnology & Advanced Materials

80 Rapidly increasing fields, such as systems biology, require the development and implementation of new technologies, enabling high-throughput and highfidelity measurements of large systems. Microfluidics promises to fulfill many of these requirements, such as performing high-throughput screening experiments on-chip, encompassing biochemical, biophysical, and cell-based assays. We design, develop and apply integrated microfluidic devices for the investigation of protein-protein interactions, gene regulation, post translational modification and for screening of cells responses to drug libraries. These devices embed thousands of micromechanical valves on a single device with a postage-sized footprint. The application of microfluidics in these research fields reduces reaction times, increases sensitivity, decreases reagent consumption and enables high- throughput research. The potential impact of microfluidics on biological sciences is significant. A number of microfluidic-based assays have already provided novel insights into the structure and function of biological systems, and the field of microfluidics will continue to impact biology. Doron Gerber’s lab specializes in microfluidics. We have developed various microfluidic devices and models for the investigation of diverse biological research fields. The projects running in our lab during 2020 include; 1) Therapeutic antibodies research conducted with Teva Pharmaceutical Industries. 2) Personalized therapy of various cancer treatments. 3) Various proteomics researches. 4) Covid 19 inhibitors screen. 5) The research of C. Elegans model through the entire life cycle. Publications 2020 and 2021 • K Shanmugha Rajan, Katerina Adler, Hava Madmoni, Dana Peleg-Chen, Smadar Cohen-Chalamish, Tirza Doniger, Beathrice Galili, Doron Gerber, Ron Unger, Christian Tschudi, Shulamit Michaeli. “Pseudouridines on Trypanosoma brucei mRNAs are developmentally regulated: Implications to mRNA stability and protein binding”. Molecular Microbiology, 2021. • Wissberg, S.; Ronen, M.; Oren, Z.; Gerber, D.; Kalisky, B. “Sensitive Readout • Aharon Gedanken, Moorthy Maruthapandi, Arumugam Saravanan, John Luong. “Antimicrobial properties of polyaniline and polypyrrole decorated with zinc-doped copper oxide nanoparticles”. Polymers 12, 1286, 2020. • Elena Benisvy-Aharonovich, Anat Zandany, Abed Saady, Yael Kinel-Tahan, Yaron Yehoshua and Aharon Gedanken. “An efficient method to produce 1,4-pentanediol from the biomass of the algae Chlorella ohadi with levulinic acid as intermediate”. Bioresource Technology Reports 11, 100514, 2020. • Hari Krishna Sadhanala, Ilana Perelshtein, Aharon Gedanken. “Boosting Electrocatalytic Hydrogen Evolution of Nickel foam Supported Nickel Hydroxide by Ruthenium Doping”. ChemistrySelect 5, 9626-9634, 2020. • Ilana Perelshtein, Nina Perkas, Shai Rahimipour, Aharon Gedanken. “Bifunctional carbondots- magnetic and fluorescent hybrid nanoparticles for diagnostic applications”. Nanomaterials 10, 1384, 2020. • Moorthy Maruthapandi, Arumugam Saravanan, John H.T. Luong, Aharon Gedanken. “Antimicrobial properties of the polyaniline composites against Pseudomonas aeruginosa and Klebsiella pneumoniae”. J. of Functional Biomaterials 11, 59, 2020. Prof. Gerber Doron The Mina & Everard Goodman Faculty of Life Sciences Member of BINA Nano-Medicine Center Research Areas • Virus host interactions • System biology • Microfluidics Abstract Integrated microfluidic applications for studying protein-protein interactions, gene regulation, intracellular mechanisms and whole cell intercellular studies. for Microfluidic High-Throughput Applications Using Scanning SQUID Microscopy”. Sci. Rep. 10 (1), 1573, 2020. • Wasserman, D.; Nachum, S.; Cohen, M.; Enrico, T. P.; Noach-Hirsh, M.; Parasol, J.; Zomer- Polak, S.; Auerbach, N.; Sheinberger-Chorni, E.; Nevenzal, H.; Levi-Dadon, N.; Xianxi, W.; Lahmi, R.; Michaely-Barbiro, E; Gerber, D.; Emanuele, M.J.; Tzur.A. “Cell Cycle Oscillators Underlying Orderly Proteolysis of E2F8”. Mol. Biol. Cell, 31 (8), 725–740, 2020. Dr. Girshevitz Olga Head of Surface Analysis Facility Member of BINA staff Publications 2020 and 2021 • Madina Telkhozhayeva, Eti Teblum, Rajashree Konar, Olga Girshevitz, Ilana Perelshtein, Hagit Aviv, Yaakov R. Tischler, and Gilbert Daniel Nessim. “Higher Ultrasonic Frequency Liquid Phase Exfoliation Leads to Larger and Monolayer to Few-Layer Flakes of 2D Layered Materials”. Langmuir, 2021. • Mirit Hen, Leroy Dokhanian, Etai Grunwald, Matan Slook, Moshe Katzman, Maayan Priel, Olga Girshevitz, and Avi Zadok. “Thin layers analysis using surface acoustic wave-photonic devices in silicon-on-insulator”. Optics Express, submit. • Lior Shani, Avital Fried, Yafit Fleger, Olga Girshevitz, Amos Sharoni and Yosef Yeshurun. “Effect of Focused Ion Beam irradiation on superconducting nanowires”. Journal of superconductivity and novel magnetism. 2021, in press. • Eitan Edri, Nina Armon, Ehud Greenberg, Shlomit Moshe-Tsurel, Danielle Lubotzky, Tommaso Salzillo, Ilana Perelshtein, Maria Tkachev, Olga Girshevitz, and Hagay Shpaisman. “Laser Printing of Multilayered Alternately Conducting and Insulating Microstructures”. Applied Materials & Interfaces, 2021. • Omer Kaspi, Olga Girshevitz, Hanoch Senderowitz. “PIXE based, Machine-

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