67 how these interact with each other. We also study the role of the non-coding genome in mediating the process of sex determination and DSD pathologies. We employ cutting-edge techniques to address these questions including CRISPR/Cas9 genome editing, transgenic mice production, advanced sequencing techniques as well as microscopy and molecular biology. Developing an in vitro model to study the gonads To develop an in vitro system that allows to model the mammalian gonads, we use mouse Embryonic Stem Cells (ESC) and develop differentiation protocols towards gonadal cell type of the gonads. In addition, we use similar protocols with Human ESC and induced Pluripotent Stem Cells to model DSD patients in vitro. Furthermore, we perform tissue engineering to model the testis and the spermatogenesis process using these stem cell-derived somatic cells along with germ cells in a 3D culture system. Publications 2021 and 2022 • Rajini Sreenivasan, Nitzan Gonen, Andrew Sinclair. “SOX Genes and Their Role in Disorders of Sex Development”. Sexual Development, 1-12, 2022. • Nitzan Gonen, Francis Poulat. “The Noncoding Genome in Sex Determination”. S. Karger, 2021. • Meshi Ridnik, Stefan Schoenfelder, Nitzan Gonen. “Cis-Regulatory Control of Mammalian Sex Determination”. Sexual Development, 1-18, 2021. • Francis Poulat, Nitzan Gonen. “Preface to the Special Issue on The Non-Coding Genome in Sex Determination”. Sexual Development, 2021. Prof. Goobes Gil Department of Chemistry Member of BINA Nano & Advanced Materials Center Energy Center Biomedicine Center Research Areas Biomaterials and Advanced Materials group Abstract Research in my group has been focused on revealing and explaining the fundamental interactions that underlie inorganic material formation in nature, a process known as biomineralization. We particularly make use of our expertise in solid-state NMR spectroscopy to analyze the rudimentary processes of biogenic material formation in atomic/molecular level. Unveiling the structure/activity relations in these specialized biomolecules involved in regulation of solid biomaterial formation has been particularly elusive. Using these findings, we develop new biomaterials for hard tissue applications based on rationale guidelines. We implement NMR characterization in materials research to understand interfaces between nanomaterials at great detail and employ molecular insights to design concept materials that are more environment friendly. Publications 2021 and 2022 • Ayala Amir, Amos Frumkin, Boaz Zissu, Aren M Maeir, Gil Goobes, Amnon Albeck. “Sourcing Herod the Great’s calcitealabaster bathtubs by a multi-analytic approach”. Scientific Reports, 2022. • Daniel Klose, Sahithya Phani Babu Vemulapalli, Michal Richman, Safra Rudnick, Vered Aisha, Meital Abayev, Marina Chemerovski, Meital Shviro, David Zitoun, Katharina Majer, Nino Wili, Gil Goobes, Christian Griesinger, Gunnar Jeschke, Shai Rahimipour. “Cu 2+-Induced self-assembly and amyloid formation of a cyclic d, l-α-peptide: structure and function”. Physical Chemistry Chemical Physics, 2022. • Francesco Bruno, Lucia Gigli, Giovanni Ferraro, Andrea Cavallo, Vladimir K Michaelis, Gil Goobes, Emiliano Fratini, Enrico Ravera. “Lysozyme is Sterically Trapped Within the Silica Cage in Bioinspired Silica–Lysozyme Composites: A Multi-Technique Understanding of Elusive Protein–Material Interactions”. Langmuir, 2022. • Khadiza Begam, Lilian Cohen, Gil Goobes, Barry D Dunietz. “Solvent Dependent Nuclear Magnetic Resonance Molecular Parameters Based on a Polarization Consistent Screened Range Separated Hybrid Density Functional Theory Framework”. Journal of Chemical Theory and Computation, 2022. • Noam Pinsk, Avital Wagner, Lilian Cohen, Christopher JH Smalley, Colan E Hughes, Gan Zhang, Mariela J Pavan, Nicola Casati, Anne Jantschke, Gil Goobes, Kenneth DM Harris, Benjamin A Palmer. “Biogenic guanine crystals are solid solutions of guanine and other purine metabolites”. Journal of the American Chemical Society, 2022. • Raju Nanda, Shani Hazan, Katrein Sauer, Victoria Aladin, Keren KeinanAdamsky, Björn Corzilius, Ron Shahar, Paul Zaslansky, Gil Goobes. “Molecular differences in collagen organization and in organic-inorganic interfacial structure of bones with and without osteocytes”. Acta Biomaterialia, 2022. • Shaul Bublil, Nicole Leifer, Raju Nanda, Yuval Elias, Miryam Fayena-Greenstein, Doron Aurbach, Gil Goobes. “Alumina thin coat on pre-charged soft carbon anode reduces electrolyte breakdown and maintains sodiation sites active in Na-ion battery–Insights from NMR measurements”. Journal of Solid State Chemistry, 2021. • Nurit Adiram-Filiba, Eli Ohaion, Gilit Verner, Avital Schremer, Merav NadavTsubery, Tammy Lublin-Tennenbaum, Keren Keinan-Adamsky, Massimo Lucci, Claudio Luchinat, Enrico Ravera, Gil Goobes. “Structure and Dynamics Perturbations in Ubiquitin Adsorbed or Entrapped in Silica Materials Are Related to Disparate Surface Chemistries Resolved by Solid-State NMR Spectroscopy”. Biomacromolecules, 2021. • Santosh Kumar, Nurit Adiram-Filiba, Shula Blum, Javier Arturo Sanchez-Lopez, Oren Tzfadia, Ayelet Omid, Hanne Volpin, Yael Heifetz, Gil Goobes, Rivka Elbaum. “Corrigendum to: Siliplant1 protein precipitates silica in sorghum silica cells”. Journal of experimental botany, 2021. • Hadar Sclar, Sandipan Maiti, Nicole Leifer, Noam Vishkin, Miryam FayenaGreenstein, Meital Hen, Judith Grinblat, Michael Talianker, Nickolay Solomatin,
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