2022 ANNUAL REPORT

89 RNA accumulates in stress granules formed in cancerous patient-derived lung organoids under arsenite treatment. RNAcyan; SGs-magenta. Cell outline- green. Publications 2021 and 2022 · Avital Schwed-Gross, Hila Hamiel, Gabriel P Faber, Mor Angel, Rakefet Ben-Yishay, Jennifer IC Benichou, Dana Ishay-Ronen, Yaron Shav-Tal. “Glucocorticoids enhance chemotherapy-driven stress granule assembly and impair granule dynamics, leading to cell death”. Journal of Cell Science, 2022. · Gabriel P Faber, Shani Nadav-Eliyahu, Yaron Shav-Tal. “Nuclear speckles–a driving force in gene expression”. Journal of Cell Science 135 (13), jcs259594, 2022. · Luna Tammer, Ofir Hameiri, Ifat Keydar, Vanessa Rachel Roy, Asaf AshkenazyTitelman, Noélia Custódio, Itay Sason, Ronna Shayevitch, Victoria RodríguezVaello, José Rino, Galit Lev Maor, Yodfat Leader, Doha Khair, Erez Lieberman Aiden, Ran Elkon, Manuel Irimia, Roded Sharan, Yaron Shav-Tal, Maria Carmo-Fonseca, Gil Ast. “Gene architecture directs splicing outcome in separate nuclear spatial regions”. Molecular Cell, 2022. · Noa Dahan, Yury S Bykov, Elizabeth A Boydston, Amir Fadel, Zohar Gazi, Hodaya Hochberg-Laufer, James Martenson, Vlad Denic, Yaron Shav-Tal, Jonathan S Weissman, Naama Aviram, Einat Zalckvar, Maya Schuldiner. “Peroxisome function relies on organelle-associated mRNA translation”. Science Advances, 2022. · Daniela Dünn-Kittenplon, Asaf Ashkenazy-Titelman, Inna Kalt, Jean-Paul Lellouche, Yaron Shav-Tal, Ronit Sarid. “The Portal Vertex of KSHV Promotes Docking of Capsids at the Nuclear Pores”. Viruses, 2021. of Interconnected Neurons”. Journal of Visualized Experiments: Jove, 2021. · Ilan Hakimi, Naor Vardi, Amos Sharoni, Michael Rosenbluh, Yosef Yeshurun. “Enhancement of photon detection in superconducting nanowire single photon detector exposed to oscillating magnetic field”. Applied Physics Letters, 2021. · Lior Shani, Philip Tinnefeld, Yafit Fleger, Amos Sharoni, Boris Ya Shapiro, Avner Shaulov, Oleg Gang, Yosef Yeshurun. “DNA origami based superconducting nanowires”. AIP Advances, 2021. Prof. Shav-Tal Yaron Dean of The Mina & Everard Goodman Faculty of Life Sciences Member of BINA Medicine Center Research Areas • Gene expression control in live cells • Live-cell imaging and RNA dynamics • Nuclear structure and function Abstract The studies in the Shav-Tal research group focus on fundamental regulatory points in the gene expression pathway and have centered on the mRNA molecule. We have examined co-transcriptional processing of mRNA at the site of transcription and its coupling to transcription; followed the dynamics of signaling pathways as they converge at the promoter and activate transcription; quantified the dynamic travels of mRNPs from the gene throughout the nucleoplasm and export through the NPC; and examined the fate of mRNA in the cytoplasm in P bodies and stress granules. We use live-cell measurements performed on fluorescence microscopes and include quantifications of the measured processes using singlemolecule analysis and mathematical modeling. Prof. Shefi Orit Faculty of Engineering Member of BINA Medicine Center Research Areas • Neurobiological systems development: image processing and network analysis • Tissue Engineering: Developing skin grafts that enable reinnervation and regeneration • Developing devices for reagents delivery into live tissue at a microscopic resolution • Neuroprosthetic devices: Neuron-Chip interface Abstract Neuroengineering and Regeneration In our lab we aim to reveal basic mechanisms underlying neuronal development and network formation and to leverage this knowledge towards obtaining improved methods for promoting neuronal regeneration. Neurons develop into a wide-variety of shapes with important implications for neuronal function and connectivity. Following nerve injuries, the process of spontaneous nerve regeneration is incomplete. We develop engineered platforms at the micro and nano scale to support physically and chemically neuronal growth. We analyze the neuronal responses by implementing morphological measurements, electrophysiological protocols, simulations and bioinformatics. Moreover, in order to target the injured nervous system, we develop drug-delivery technologies. The image shows florescent NPs injected into cells in the leech CNS ganglion via an electrode (shown near the orange injected neuron).

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