93 Prof. Weiss Shimon Department of Physics BIU Department of Chemistry and Biochemistry UCLA Member of BINA Nano-Electro Magnetism & Spintronics Center Research Areas • Single molecule detection and spectroscopy • Dynamic structural/molecular biology • Protein folding, protein-protein and protein-DNA interactions • Novel Bio-Nano-Technology probes semiconductor nanocrystals, quantum dots, semiconductor nanocrystals voltage sensors • Fluorescence microscopy/spectroscopy super resolution microscopy • Mesoscopic systems Abstract Voltage Sensing Nano Particle as an Optical Tool for Electrical Potential Activity in Neurons Voltage sensing nanoparticles (vsNPs) have been developed for non invasive optical recording of membrane potential at the single particle and nanoscale level, at multiple sites, in a large field-of-view. vsNPs allow for ‘pointy’, single particle voltage detection. However, they still face challenges and additional improvements are needed before this new generation of sensors can be widely translated to neurophysiological applications. We developing targetable membrane potential nanosensors (MPNs) that optically and non-invasively record MPs at multiple sites in a large field of-view (FoV) of primary cultured neurons with single-particle brightness and voltage sensing capacity at the nanoscale. We searching for the ultimate nanometer size particle suitable for site specific voltage membrane sensing on the nanoscale. We therefore studied and developing different systems. Single-particle membrane potential optical recordings from NDTF,Cy5-Ab-avidin complex targeting GABAAR and DPA in cultured cortical neurons. Publications 2022 and 2023 • Subhabrata Ghosh, Ulrich Ross, Anna M Chizhik, Yung Kuo, Byeong Guk Jeong, Wan Ki Bae, Kyoungwon Park, Jack Li, Dan Oron, Shimon Weiss, Jorg Enderlein, Alexey I Chizhik. “Excitation Intensity-Dependent Quantum Yield of Semiconductor Nanocrystals”. The Journal of Physical Chemistry Letters, 2023. • Antonino Ingargiola, Shimon Weiss, Eitan Lerner. “Monte Carlo Diffusion-Enhanced Photon Inference: Distance Distributions and Conformational Dynamics in SingleMolecule FRET”. Journal of Physical Chemistry B, 2023. • Ganesh Agam, Christian Gebhardt, Milana Popara, Rebecca Mächtel,J ulian Folz, Benjamin Ambrose, Neharika Chamachi, S ang Yoon Chung, Timothy D Craggs, Marijn de Boer, Dina Grohmann, Taekjip Ha, Andreas Hartmann, J elle Hendrix, Verena Hirschfeld, Christian G Hübner, Thorsten Hugel, Dominik Kammerer, Hyun-Seo Kang, Achillefs N Kapanidis, Georg Krainer, Kevin Kramm, Edward A Lemke, E itan Lerner, Emmanuel Margeat, Kirsten Martens, Jens Michaelis, J aba Mitra, Gabriel G Moya Muñoz, Robert B Quast, Nicole C Robb, Michael Sattler, Michael Schlierf, Jonathan Schneider, Tim Schröder, Anna Sefer, Piau Siong Tan, Johann Thurn, Philip Tinnefeld, John van Noort, Shimon Weiss, Nicolas Wendler, Niels Zijlstra, Anders Barth, Claus AM Seidel, Don C Lamb, Thorben Cordes. “Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins”. Nature Methods, 2023. • Debjit Roy, Xavier Michalet, Kiran Bharadwaj, Evan W Miller, Yijie Wang, Arjun Deb, Michael A Wayne, Claudio Bruschini, Edoardo Charbon, Mahbanoo Vakili, Robert Gunsalus, Robert T Clubb, Shimon Weiss. “Towards precise optical measurements of steady state of and small changes in resting membrane potentials”. Biophysical Journal, 2023. • Yuting Miao, Shimon Weiss, Xiyu Yi. “PySOFI: an open source Python package for SOFI”. Biophysical Reports, 2022. • Yuting Miao, Robert C Boutelle, Anastasia Blake, Vigneshwaran Chandrasekaran, Chris J Sheehan, Jennifer Hollingsworth, Daniel Neuhauser, Shimon Weiss. “Super-resolution Imaging of Plasmonic Near-Fields: Overcoming Emitter Mislocalizations”. The Journal of Physical Chemistry Letters, 2022. • Arkaprabha Basu, Manash K Paul, Shimon Weiss. “The actin cytoskeleton: Morphological changes in pre-and fully developed lung cancer”. Biophysics Reviews, 2022. • Arkaprabha Basu, Manash K Paul, Mitchel Alioscha-Perez, Grosberg Anna, Hichem Sahli, Steven M Dubinett, Shimon Weiss. “Statistical Parametrization of Cell Cytoskeleton (SPOCC) reveals novel lung cancer cytoskeletal phenotype with partial EMT signature”. Communications Biology, 2022. • Jenjamin Ambrose, Victoria E Hill, Robert A Shaw, Tristan Johnston-Wood, Matthew Willmott, Callum Johnston, Rebecca Mächtel, Thorben Cordes, Eitan Lerner, JG Hill, Shimon Weiss, Timothy D Craggs. “A novel single molecule fluorescence quenching technique for measuring distances below 3 nm”. Biophysical Journal, 2022. • Debjit Roy, Xavier Michalet, Evan W Miller, Robert P Gunsalus, Robert T Clubb, Shimon Weiss. “Bacterial resting membrane potential: a case study with Bacillus subtilis”. Biophysical Journal, 2022. • Debjit Roy, Zehavit Shapira, Shimon Weiss. “Membrane potential sensing: Material design and method development for single particle optical electrophysiology”. The Journal of Chemical Physics, 2022. • Jason T Smith, Alena Rudkouskaya, Shan Gao,Arin Ulku, Claudio Bruschini, Edoardo Charbon, Shimon Weiss, Margarida MR Barroso, Xavier Intes, Xavier Michalet. “Characterization of a large gated SPAD array for widefield NIR fluorescence lifetime imaging in vitro and in vivo”. Biophysical Journal, 2022. • Jason T Smith, Alena Rudkouskaya, Shan Gao, Juhi M Gupta, Arin Ulku, Claudio Bruschini, Edoardo Charbon, Shimon Weiss, Margarida Barroso, Xavier Intes, Xavier Michalet. “In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera”. Optica, 2022.
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